Propagation of goat pox virus on monolayer cell cultures
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Propagation of goat pox virus on monolayer cell cultures.
Although the majority of different members of poxviruses have been carefully studied, !ittle is known about goat pox and no detailed report on the disease and its causative agent is, so far, available. The cultivation of goat pox virus (GPV) in cell cultures reported in the present paper makes available the most reliable and economic source for large s~ale produc~ion of vaccine to control the d...
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Kid kidney cells form less than 2 months of age were grown, 80% confluent monolayers were obtained after 10-12 days. Live attenuated goat pox virus vaccine (Gorgan 55, Iranian) was used to infect the confluent monolayers. Eighty percent cytopethic effect (CPE) was obtained on day 5 of virus inoculation. TCID50 calculated for the pool virus was 10 / ml. Vaccine was found free from any bacterial ...
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This study demonstrates that the bovine leukemia virus (BLV) can infect in vitro cells of human, simian, bovine, canine, caprine, ovine, and bat origin. Cultures of these cells, cocultivated with BLV-infected cells or inoculated with cell-free BLV preparations, continuoously showed the presence of cells with the internal BLV antigen as well as BLV-induced syncytia. Virus replication was abundan...
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BACKGROUND: Caprine pox virus disease play an important role in major constraints to small ruminants production in Iran. OBJECTIVES: This study was conducted in different districts of Iran to determine the prevalence of goats pox virus disease in different age groups, sex and seasons in Black goats of Iran. METHODS: In this study skin lesions of male and female goats were collected randomly ...
متن کاملDetection, identification, and differentiation of sheep pox virus and goat pox virus from clinical cases in Giza Governorate, Egypt
AIM To isolate, identify, and differentiate Capripoxviruses (CaPV) (sheep pox virus and goat pox virus) infections by egg inoculation, transmission electron microscopy (TEM), and 30 kDa RNA polymerase subunit gene-based polymerase chain reaction (PCR) (RPO30) in clinically affected animals in Hawamdia township of Giza Governorate, Egypt. MATERIALS AND METHODS A total of 37 scab samples were c...
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عنوان ژورنال
دوره 20 شماره 1
صفحات 91- 95
تاریخ انتشار 1968-02-01
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